The pupal cuticle of Drosophila: differential ultrastructural immunolocalization of cuticle proteins.
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چکیده
منابع مشابه
The pupal cuticle of Drosophila: differential ultrastructural immunolocalization of cuticle proteins
Precise ultrastructural localization of Drosophila melanogaster pupal cuticle proteins (PCPs) was achieved by the immunogold labeling of frozen thin sections. PCPs were found in lamellate cuticle and intracellular vesicles but, curiously, were absent from the assembly zone of the cuticle. Antibodies that distinguish between the two classes of PCPs--low molecular weight (L-PCPs) and high molecul...
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We investigated the synthesis and localization of Drosophila pupal cuticle proteins by immunochemical techniques using both a complex antiserum and monoclonal antibodies. A set of low molecular weight (15,000-25,000) pupal cuticle proteins are synthesized by the imaginal disk epithelium before pupation. After pupation, synthesis of the low molecular weight proteins ceases and a set of unrelated...
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Three proteins, MsCP20, MsCP27 and MsCP36, that are secreted in greatest quantity into the pharate pupal cuticle of Manduca sexta ( Hopkins et al., 2000) were purified and their amino acid sequences determined by mass spectrometry and Edman degradation. Although these proteins become sclerotized and insoluble in the pupal exoskeleton, their sequences contain features characteristic for proteins...
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Proteins in pupal abdominal cuticle of the tobacco hornworm, Manduca sexta, were characterized during the pre-ecdysial and post-ecdysial periods of sclerotization and endocuticle formation. Protein extractability decreased dramatically as the cuticle became sclerotized through 6 h post-ecdysis, but increased rapidly from 9 to 48 h as endocuticular layers were secreted. Nearly 100 proteins that ...
متن کاملThe cuticle.
The nematode cuticle is an extremely flexible and resilient exoskeleton that permits locomotion via attachment to muscle, confers environmental protection and allows growth by molting. It is synthesised five times, once in the embryo and subsequently at the end of each larval stage prior to molting. It is a highly structured extra-cellular matrix (ECM), composed predominantly of cross-linked co...
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ژورنال
عنوان ژورنال: The Journal of Cell Biology
سال: 1986
ISSN: 0021-9525,1540-8140
DOI: 10.1083/jcb.102.1.306